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Image Search Results
Journal: mBio
Article Title: The Synthetic Phenotype of Δ bamB Δ bamE Double Mutants Results from a Lethal Jamming of the Bam Complex by the Lipoprotein RcsF
doi: 10.1128/mBio.00662-19
Figure Lengend Snippet: Quantitative proteomic analysis of Δ bamB Δ bamE mutant. Volcano plot of protein expression comparison between wild-type and Δ bamB Δ bamE cells. Each dot represents a protein. The horizontal axis shows the log 2 fold change of protein abundance. The vertical axis represents the likelihood that differential expression is observed between the two conditions . Red dots represent β-barrel OMPs, and green dots indicate proteins involved in the Rcs stress regulon that confidently (>0.95) change their respective expression levels. Global levels of β-barrel OMPs are reduced while levels of Rcs regulon members are mostly upregulated in the Δ bamB Δ bamE mutant relative to wild type, with a high degree of confidence.
Article Snippet: The native promoter, defined as 1,000 bp upstream of bamA , and the bamA open reading frame were amplified from a
Techniques: Mutagenesis, Expressing
Journal: mBio
Article Title: The Synthetic Phenotype of Δ bamB Δ bamE Double Mutants Results from a Lethal Jamming of the Bam Complex by the Lipoprotein RcsF
doi: 10.1128/mBio.00662-19
Figure Lengend Snippet: Quantitative analysis of global OMP levels. (A) The relative fold change of β-barrel OMPs comparing the Δ bamB Δ bamE , Δ bamB Δ bamE Δ rcsF , Δ bamB Δ bamE bamA F494L , and Δ bamB Δ bamE Δ rcsB mutants to the wild type is shown. Whiskers indicate 95% confidence intervals. The Δ bamB Δ bamE double mutant exhibits a universal defect in OMP levels with respect to the wild-type control. OMP levels are similarly reduced in the Δ bamB Δ bamE Δ rcsB triple mutant. Both Δ rcsF and bamA F494L restore OMP levels, to similar degrees. (B) Projecting the OMP protein abundance changes for various mutants on the vector defined by the differences between the wild type and Δ bamB Δ bamE double mutant indicates that Δ bamB Δ bamE Δ rcsB does not rescue the phenotype of the double mutant, but both Δ bamB Δ bamE Δ rcsF and Δ bamB Δ bamE bamA F494L partially rescue the OMP expression phenotype.
Article Snippet: The native promoter, defined as 1,000 bp upstream of bamA , and the bamA open reading frame were amplified from a
Techniques: Mutagenesis, Plasmid Preparation, Expressing